In conclusion, our results showed that gene therapy based in recombinant AdTNF, reactivates protective immune responses by increasing IFN-γ and iNOS expression in inflammatory cells located in pneumonic areas, which leads to a better formation of granulomas that efficiently contain and eliminate Mtb, resulting in lesser bacterial load and lung damage (percentage of pneumonia) that leads to total survival of mice. Here, NOS2 is linked to susceptibility to pneumonia measurement.