CASP3 and infection: Cell apoptosis is induced by various biotic factors, such as bile acid disorder [37], and various abiotic factors, such as Carnitine [38], alcohol, radiotherapy, severe burns [39], etc. Previous studies of Caspase 3 in aquatic organisms revealed that in grass carp (Ctenopharyngodon idella) [40] and turbot (Scophthalmus maximus (L.)[41] long-term stress and pathogen infection could both bring about organ damage by inducing an apoptosis response through a significant increase in the expression of caspase 3 in the spleen.