Furthermore, in liver oligometastasis from colorectal cancer, a high intratumoral CD8/CD3 ratio (>0.32; significant stained cells were quantified by identifying and calculating subtype-specific cell numbers per field based on positivity and total cell count through four-color IHC) correlated with significantly longer 3-year relapse-free survival and 3-year overall survival rates than those with low intratumoral CD8/CD3 ratios [62]. This evidence concerns the gene CD8A and colorectal cancer.