To verify the dependability of the RNA-seq, the expression profile of 11 DEGs (WNT7A, WNT7B, WNT16, FZD1, FZD8, FZD9, FGFR1, NTRK2, SFRP1, ITGA5, and CAMK2B) and 11 known miRNAs (let-7d, let-7e, miR-31-5p, miR-34b-5p, miR-125a-3p, miR-143-3p, miR-149-5p, miR-193a-5p, miR-324-3p, miR-362-5p, and miR-877-5p) associated with HF development was explored using RT-qPCR (Figure 6 and Figure 7). This evidence concerns the gene CAMK2B and hydrops fetalis.