For instance, in lean and obese diabetic Zucker diabetic fatty rats (a model of type 2 diabetes), CL 316,243 infusion revealed an anti-obesity and anti-diabetes effect by improving glucose tolerance, insulin sensitivity, thermogenesis, mitochondrial biogenesis, WAT, BAT, and skeletal muscle glucose uptake and reducing plasma FFA levels [163]. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.