Assay of all other sampled breast cancer cases in the pedigree in which the four sequenced MDH2- and TENM2- and CCDC136-variant-carrying recurrent breast cancer cases were originally identified (Figure 1) found one additional breast cancer case carrier of the TENM2 variant, two additional breast cancer case carriers of the CCDC136 variant, and seven additional carriers of the MDH2 variant (one inferred). Here, CCDC136 is linked to breast cancer.