We grafted the four Panc-1 cell lines subcutaneously into nude mice and monitored the tumor size weekly for 10 weeks, either by using a caliper to establish a growth curve within a given time frame or by measuring mCherry intensity in the tumor area (for Panc-1-mCherry or Panc-1-mCherry-TRPV6 cells) (Figure 9A). Here, TRPV6 is linked to neoplasm.