FOXO1 and cancer: Similarly, HTH-01-015 reduced cell viability in spheroid cultures from MDA-MB-231, U87, and DLD-1 cells (Fig. 8P), suggesting a critical role of NUAK1 in regulating cancer cell survival via Akt/FOXO1/3a axis upon growth factors stimulation and, thus, NUAK1 inhibition is a potential therapeutic approach under specific cellular contexts.