However, while the effect of knocking down NeuroD1 with shRNA expression vectors in HCC cells was significant, it is noteworthy that the expression of NeuroD1 was not totally abolished; hence, it is plausible that NeuroD1 also regulate other ferroptosis-related factors, although less significant compared to its regulation on GPX4. This evidence concerns the gene NEUROD1 and hepatocellular carcinoma.