Knocking down NeuroD1 significantly suppressed the viability (Figs 1B and S2A) and colony formation potential (S2B and S2C Fig) of HCC-LM3 and MHCC-97H cells, while NeuroD1 overexpression robustly increased them (S2D–S2G Fig). Here, NEUROD1 is linked to hepatocellular carcinoma.