Moreover, re-expressing NeuroD1 in NeuroD1-knocked down HCC-LM3 cells abolished the increase in lipid ROS, mitochondrial ROS, MDA, 4-HNE, and LDH levels caused by NeuroD1 knockdown to the levels similar to those of control (S5A–S5E Fig). The gene discussed is NEUROD1; the disease is hepatocellular carcinoma.