In line with these data, as shown in Fig 3, we could show that the induction of T2D in female rats significantly reduced the cardiac levels of Sirt1 (P ≤ 0.01), Sirt2, Sirt3, and Sirt6 proteins (P ≤ 0.05) compared with the Sh-Ctl group. This evidence concerns the gene SIRT6 and type 2 diabetes mellitus.