The results of this study showed that the induction of T2D by the HFD-STZ method reduces the expression of Sirt1, Sirt2, Sirt3, and Sirt6, and when we induced menopause in T2D animals, a further decrease was observed in Sirt1, Sirt3, and Sirt6 levels. Here, SIRT1 is linked to type 2 diabetes mellitus.