In order to further study the regulatory effect of AsC on DC maturation, based on a previous study [20], we isolated bone marrow from the femurs of C57BL/6 mice, used cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL4) to induce their differentiation into DCs for 6 days, and then co-cultured them with tumor cells from different treatment groups for 24 h (Figure 7C). This evidence concerns the gene CSF2 and neoplasm.