Taking into account the long-history of the KC concept, we performed a special study to verify our hypothesis that the R. temporaria KC is a special structural compartment of the GV, which may demonstrate some reminiscence of the insect KC and/or the NE, and is involved in the regulation of the spatial organization of diplotene bivalents, gathering into the karyosphere after the natural detachment of oocyte chromosomes from the NE. Here, CALCA is linked to keratoconus.