Based on this, this study aims to further investigate the role of WEPO in inhibiting PEDV infection through a stable infection mode in vitro, the role of WEPO in inhibiting PEDV replication and its impact on the Caspase-1/GSDMD pyroptotic pathway, followed by the role of Caspase-1/GSDMD in the inhibition of PEDV by WEPO. This evidence concerns the gene GSDMD and infection.