In the APOE gene, DNA methylation at two CpG sites (3/13) that are known to show age-dependent changes was related with the total cholesterol and high-density lipoprotein cholesterol ratio, but not with cognitive status, family history of AD, or the risk of cardiovascular disease in a blood-based DNA methylation study of 5828 people from the Generation Scotland cohort [89]. The gene discussed is APOE; the disease is cardiovascular disorder.