Among these pathways, the pathways related to cell malignization dominated in the pathways list for hepatocyte-derived malignant cells (‘RHO GTPase cycle’, ‘p53 downstream pathway’, ‘regulation of MAPK cascade’, and ‘signaling by BRAF and RAF1 fusion’ for HepG2 cells and ‘transcriptional misregulation in cancer’, ‘cell fate commitment’, and, to some extent, ‘response to UV’ for Huh7 cells). The gene discussed is RAF1; the disease is cancer.