Functional annotation clustering of the negative selection sweeps for Crossbred-HF depicted enriched KEGG terms (enrichment score: 1.22) including ovarian steroidogenesis (bta04913), prolactin signaling pathway (bta04917), cortisol synthesis and secretion (bta04927), steroid hormone biosynthesis (bta00140) and Cushing syndrome (bta04934) (Supplementary Table S1). The gene discussed is PRL; the disease is Cushing syndrome.