Our study has shown that a single subcutaneous injection of 300 μM Cx43asODN under the insult resulted in the reduction of the pathological increase of epidermal Cx43 protein levels, neutrophil infiltration, epidermal HMGB1 and epidermal RIP3 in an experimental early PU model of a single pinch of 1.5 h of ischaemia. The gene discussed is HMGB1; the disease is ischemia.