The morphology of the spindle shape with long-armed intercellular connections in CytoMix-treated KCs and the polygonal shape in control KCs is shown in Figure 2C. Using real-time RT-PCR, we detected increased VIM mRNA expression levels in CytoMix-treated KCs compared to the controls at 48 and 72 h post-treatment (Figure 2D). This evidence concerns the gene VIM and dry eye syndrome.