1) The drug MNPC inhibits NQO1, leading to increased ROS levels and promoting GBM cell death.2) C/EBPβ can regulate the transcription of NQO1, neutralize ROS in GBM cells, and promote proliferation.3) NQO1 can also bind with the substrate TSB, resulting in the significant generation of ROS, and promoting GBM cell death. This evidence concerns the gene NQO1 and glioblastoma.