GPX4 and infection: Macrophage necrosis after infection is the main cause of bacterial spread and disseminated disease.[42] Mtbinduced macrophage necrosis is associated with reduced levels of glutathione and GPX4, along with increased free iron, lipid peroxidation, and mitochondrial superoxide, all of which are characteristic marks of ferroptosis.[43] Moreover, necrosis in infected macrophage cultures was well suppressed by ferrostatin-1, a well-characterized ferroptosis inhibitor.