In the third batch of AAV, we produced AAV9-TTR-cohFIX, which is a codon-optimized construct encoding human factor IX (hFIX) for treating hemophilia B. The hemophilic B mouse model, C3H/HeJ-HB, was used to test the functionality of the differently purified vectors (Figure 8(a)). Here, F9 is linked to hemophilia B.