USO1 and acute lymphoblastic leukemia: For instance, Jaiswal et al. conducted a CRISPR/Cas9 screening on MLL-rearranged B-ALL positive (SEM) and negative (Nalm-6) cell lines to identify dependencies on several genes, including EIF3E, EPRS, and USO1. To validate the screening results, a specific CRISPR/Cas9-mediated knockout of USO1 was generated in MLL-translocated B-ALL cells.