To confirm the role of PTP1B in BACH1-mediated insulin resistance on HFD in vivo, we silenced PTP1B by administering AAV of the 8-serotype containing a construct encoding murine PTP1B (Ptpn1 gene) short hairpin RNA (shRNA) under the control of TBG promoter for hepatocyte-specific expression (AAV-shPtpn1) to selectively inhibit PTP1B in hepatocytes in vivo. Here, PTPN1 is linked to Insulin resistance.