This study observed that the fluorescence intensity of the Notch overexpression group was significantly lower than that of the Model control group, while the fluorescence intensity of VE-Cadherin and Zo-1 protein in the low-expression group was higher than that of the Model group, suggesting that Notch signal expression significantly inhibited the expression of VE-Cadherin and Zo-1 protein during ALI, thereby increasing the permeability of pulmonary capillary barrier. The gene discussed is TJP1; the disease is acute respiratory distress syndrome.