NFKB1 and colorectal carcinoma: Importantly, this HEK cell line lacks expression of TLR2 and TLR4, which are linked to F. nucleatum’s major activity in CRC (Figure 1b).18,19,24,34,44,45 To investigate whether the remaining TLRs expressed in HEK-NF-κB were responsible for the NF-κB response to F. nucleatum supernatants, we deleted the gene encoding the adaptor protein MYD88 (MYD88-/-), a key protein in TLR signaling pathways.