To gain insight into how silencing VPS4A could affect other ESCRT components and ASFV infection, we focus on HRS (ESCRT-0) and the adaptor protein ALIX, a crucial player for several virus models to pursue a successful infection (Le Blanc et al., 2005; Munshi et al., 2007; Pattanakitsakul et al., 2010; Pasqual et al., 2011; Jiang et al., 2020). Here, PDCD6IP is linked to infection.