Our results would support that ASFV needs an efficient cholesterol transport that depends on LBPA and NPC proteins from early steps of the infection, while the LBPA–ALIX interaction, related to ILV formation and back fusion, would remain in the background, since ASF virions fuse with the LE (Cuesta-Geijo et al., 2012; Hernaez et al., 2016). The gene discussed is PDCD6IP; the disease is infection.