In this large-scale epidemiological study based on data from various anthropometric and biochemical measurements taken during health examinations of individuals at the Murakami Memorial Hospital of Gifu, after adjusting for several confounding factors such as demographic characteristics, lifestyle, physical measurements, and laboratory biochemical indicators, higher liver enzymes (including ALT, AST, and GGT) were independently associated with both male and female NAFLD. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.