In male participants, ALT exhibited the strongest association with NAFLD (OR=2.19, 95% CI: 2.01-2.39), followed by AST (OR=1.38, 95% CI: 1.28-1.49) and GGT (OR=1.14, 95% CI: 1.08-1.20). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.