The identified PhenoGraph clusters were assigned to seven major populations (Figures 2B, S1): tumor cells (panCK+), CD4+ T cells (CD45+CD3+CD4+), CD8+ T cells (CD45+CD3+CD8+), B cells (CD45+CD20+), blood vessel (CD31+ and/or aSMA+), fibroblasts (collagen+), and myeloid cells (CD45+CD14+). This evidence concerns the gene CD8A and neoplasm.