More detailed phenotyping of subpopulations was subsequently performed by iterating PhenoGraph clustering on three of the aforementioned major subsets, namely CD4+ T cells (regulatory = FoxP3+, naive = CD45RA+, memory = CD45RA−), tumor cells (proliferating = Ki67+, non-proliferating = Ki67-), and myeloid cells (M1 macrophages = CD68+CD206-, M2 macrophages = CD68+CD163+CD206+, monocytes = CD14+CD68low, granulocytes = CD14lowCD11b+Granzyme B+, dendritic cells = CD11c+, myeloid derived suppressor cells [MDSCs] = CD14+CD11b+HLA-DR-, Figure 2C, see STAR Methods). Here, ITGAX is linked to neoplasm.