Sera from TB patients, IGRA+ household contacts, and IGRA- BCG vaccinated healthy controls all reacted to all three proteins; however, PPE26 induced a weak antigen-specific IgM antibody response in IGRA+ household contacts (Figures S3B), while PE31 showed highest IgM antibody titres in this same group, resulting in significant differences (p=0.0286) when compared to PPE26 (Figures S3-B). This evidence concerns the gene CD40LG and tuberculosis.