NLRC4 and infection: However, at 96 h post-infection Naip1-7-/- and Nlrc4-/- PMs were more permissive to T. cruzi replication than C57BL/6 WT cells, presenting higher frequency of infected cells (Figure 1C) and higher numbers of intracellular amastigotes (Figures 1D, E) in comparison to their littermate control cells.