PTEN and intrahepatic cholangiocarcinoma: To explore the molecular mechanism of PTEN in ICC, LV vectors were used to construct HuCCT1 cells for stably expressing PTEN (PTEN-EXO) or CRISPR/Cas9-based (PTEN-KO), and the empty vector (PTEN-EXO NC) and NC LV (PTEN-KO NC) were used as controls.