Mice carrying a knock-in mutation of Nlrp3 (R258W), which corresponds to a causal mutation in cryopyrin-associated periodic syndrome patients28, were crossed with Zbtb16-/- mice to generate animals expressing hyper-active NLRP3 in normal or Zbtb16-deficient backgrounds. This evidence concerns the gene ZBTB16 and cryopyrin-associated periodic syndrome.