We performed orthotopic implantation of Enpp1-knockout 4T1 murine breast cancer cells overexpressing either WT ENPP1 (ENPP1WT-OE) or catalytically dead ENPP1 (ENPP1T238A-OE) into WT mice (SI Appendix, Fig. S1 A–C). Here, ENPP1 is linked to breast cancer.