In line with previous studies (43, 44), we found a significant increase in F4/80 mRNA level along with a decrease in the gene expression of CD11c in the HFD group compared to the SCD group, suggesting that HFD-induced obesity augmented macrophage infiltration, which was reversed following the supplementation of B. coagulans T4 and L. paracasei TD3, suggesting that these probiotics attenuated inflammation in the skeletal muscle tissue. The gene discussed is ITGAX; the disease is obesity due to melanocortin 4 receptor deficiency.