SLC12A3 and Hypokalemia: This gene encodes the thiazide-sensitive sodium-chloride cotransporter (NCCT), a protein situated on the apical membrane of the distal convoluted tubule in the kidney.[14] Functional loss mutations within SLC12A3 lead to structural and/or functional anomalies of NCCT, disrupting sodium-chloride reabsorption in the distal convoluted tubule,[15] subsequently triggering a series of pathophysiological and clinical manifestations, including hypovolemia, RAAS activation, hypokalemia, and metabolic alkalosis.