Subgroup analysis based on specific glycolysis markers revealed that higher expression of PKM2 (P < 0.001), STMN1 (P = 0.002), MCT4 (P < 0.001), GLUT1 (P = 0.025), HK-2 (P < 0.001), and CA9 (P < 0.001) was significantly correlated with poor OS in liver cancer. The gene discussed is HK2; the disease is liver cancer.