CD4 and neoplasm: Using a 23-marker spectral flow analysis and established dimensionality reduction and clustering tools, we identified T cells with hallmarks of prior antigen exposure (CCR7-, CD45RA-) and tissue residency (CD103+)25 in both the CD4 (cluster CD4 P1) and CD8 (clusters CD8 P7and P8) compartments, suggesting tumor-experienced T cells were recirculating into the blood (Fig. 3A, F).