PCK2 and infection: At day 60 post-infection, mice were challenged with either PBS, 5 × 106 heat-killed wildtype L. major promastigotes or 5 × 106 heat-killed PEPCK-deficient L. major promastigotes20 via footpad injection13, and intravital microscopy of the previously healed ear dermis was performed after 2 weeks (Fig. 5A).