We found that ESRP1 drives CLSTN1 exon 11 splicing, resulting in the spliced CLSTN1 (short CLSTN1), which participates in inhibiting tumor cell EMT and stabilizing Ecadherin/β-catenin adhesive structure, which is an important mechanism for inhibiting gastric cancer metastasis. This evidence concerns the gene CLSTN1 and gastric cancer.