In addition, we examined the effect of CLSTN1 on key EMT proteins and found that CLSTN1-S promoted Ecadherin protein expression and inhibited Ncadherin expression, while CLSTN1-F had the opposite effect (Fig. 6C), suggesting that CLSTN1-S may mediates ESRP1 induced inhibition of gastric cancer metastasis. This evidence concerns the gene CDH2 and gastric cancer.