These analyses revealed that MZB1 is an exclusive marker of the DCIS #1 ROI and cell type, GJB2+ stromal cells were found in the DCIS #2 ROI, ALDH1A3, KRT15, and KRT23 were highly expressed in myoepithelial cells of the DCIS #1 ROI, and the macrophage marker MMP12 was absent from the invasive ROI. Here, GJB2 is linked to ductal breast carcinoma in situ.