ITPR3 and Rotavirus infection: Together, these data show rotavirus infection produces robust Ca2+ signaling in both MA104 and HEK293 cells, and this is not decreased in the absence of IP3R. However, we found that HEK293 cells were suboptimal for examining rotavirus-induced Ca2+ signaling because the ICWs produced are smaller than those from MA104 cells, which potentially complicates quantitation of infected cell versus neighboring cell Ca2+ signals.