We infected parental MA104-GCaMP6s and MA104-GCaMP6s-IP3R-TKO cells with 25 PFU to ensure well-isolated infected cells and cultured in the presence of trypsin to visualize SA11-mRuby spread and measure the kinetics of virus replication over 96 h post-infection. The gene discussed is ITPR1; the disease is infection.