Representative images (Fig. 1D) and Ca2+ signaling traces (Fig. 1E) show that both parental HEK293-GCaMP6s and HEK293-GCaMP6s-IP3R-TKO cells exhibit robust Ca2+ signaling during rotavirus infection (red lines) that is much greater than in mock-infected cells (black lines). Here, ITPR3 is linked to Rotavirus infection.