These assays include gene mutation identification (such as microsatellite instability (MSI) and POLE),8, 15 gene expression (such as ColoPrint and Oncotype DX Colon),9, 16, 17 tumor immune microenvironment (Immunoscore),13, 14 and circulating tumor DNA (ctDNA).10, 11, 12, 18. Here, POLE is linked to neoplasm.