In Supplementary Figure 1, the results of quantitative real-time PCR suggest that notable decreases and increases were observed in the expressions of CLDN5 and TBC1D1 in the ED group compared with the control group (p-value < 0.05), emphasizing the critical genetic links of CLDN5 and TBC1D1 between ED and depression. This evidence concerns the gene CLDN5 and depressive disorder.