INS and gestational diabetes: Treg subsets:• Naive CD45RA+ Tregs: % decreased significantly in both diet-adjusted and insulin-controlled GDM• HLA-DR−CD45RA− memory Tregs (DR-Tregs): % increased significantly in diet-controlled GDM• HLA-DRlow+CD45RA−: % increased significantly in insulin-controlled GDM• HLA-DRhigh+CD45RA− memory Tregs (DRlow+ and DRhigh+ Tregs): increased significantly in insulin-controlled GDM