• TCD4+: no significant difference• TCD3+CD4+CD25+: no significant difference• CD25bright: there was a significantly lower percentage in GDM• FOXP3high: % was significantly lower in GDM• NK cells: no significant difference• CD56dim: there was a higher frequency in GDM• NKT cells: no significant difference• TNF-α production by Treg cells: higher in GDM• No significant difference was observed in other cytokines and chemokines produced by Treg and NK cells (including IL-10). This evidence concerns the gene TNF and gestational diabetes.