Of note, only AML in direct cell contact increased TNAP expression in BMSCs (direct contact vs transwell in basal conditions: + HL-60, p=0.0023; + KG-1, p=0.0153; + THP-1, p=0.01; direct contact vs transwell in osteoinductive conditions: + HL-60, p=0.0177; + KG-1, p=0.0265; + THP-1, p=0.0373) (Figures 1D, E). This evidence concerns the gene ALPL and acute myeloid leukemia.