Strikingly, treatment with DAPT successfully avoided the up-regulation of TNAP in BMSCs cocultured with AML cell lines restoring its expression to control level (DAPT vs vehicle in basal conditions: HL-60, p=0.0006; KG-1, p=0.0032; THP-1, p=0.0037; DAPT vs vehicle in osteoinductive conditions: HL-60, p=0.0305; KG-1, p=0.0164; THP-1, p=0.0155) (Figures 5C, D). The gene discussed is GLI2; the disease is acute myeloid leukemia.