Adult male and female C57BL/6J mice (3-6 months) genetically modified to contain fluorescent nicotinic acetylcholine receptors (nAChRs) (α4-mCherryα6-GFP mice) were administered intraperitoneal injection of farnesene (0.1-10 mg/kg) with or without nicotine (0.5 mg/kg); mouse neuroblastoma 2a (neuro-2a) cells transfected with α4-mCherry, α4-GFP, and β2WT or α4-mCherry, α6-GFP, and β2WT) nAChR subunits and treated with 500 nM farnesene for 24 h to examine changes in α4α6β2 and α4β2 nAChR stoichiometry. The gene discussed is CHRNA4; the disease is neuroblastoma.