RT-qPCR showed that the expression of M1 polarization markers iNOS (p < 0.01) and TNF-α (p < 0.001) increased significantly after being co-cultured with the supernatant from the AGAP2-AS1 knockdown ccRCC cells, while M2 polarization markers of Arg-1 (p < 0.01) and IL-10 (p < 0.01) was significantly reduced (Fig. 2K, L). This evidence concerns the gene ARG1 and nonpapillary renal cell carcinoma.