Similarly, flow cytometry showed that the number of CD163+ macrophages increased after co-culture with the supernatant of RCC cells overexpressing AGAP2-AS1, while co-culture of the supernatant from the AGAP2-AS1 knockdown ccRCC cells with M0 macrophages brought about opposite finding (Additional file 1: Figure S1A and B). The gene discussed is CD163; the disease is nonpapillary renal cell carcinoma.