Furthermore, compared to the wild-type BCG group, the transcriptome of the B∆tbcm group showed downregulated genes, including SLC25A26 (− 3.276-fold), NDUFA8 (− 3.324-fold) and DTYMK (− 2.931-fold); the absence of these genes is associated with mitochondrial disease (Fig. 4B) [27–29]. The gene discussed is SLC25A26; the disease is inborn mitochondrial metabolism disorder.