Among the homeobox TFs, Engrailed‐1 (EN1) is essential in the development of central nervous system and implicated in the control of cell differentiation, growth, survival, and axon guidance at the cellular level.[12, 13, 14, 15, 16] In addition, several studies have reported aberrantly expressed EN1 and the association with poor prognosis in human malignancies, including glioblastoma, salivary gland adenoid cystic carcinoma, and breast‐related cancers.[17, 18, 19, 20, 21, 22, 23] However, the detailed molecular mechanisms by which EN1 promotes PDA progression remain unknown. This evidence concerns the gene EN1 and Patent ductus arteriosus.