Through transcriptome and epigenome profiling on the paired mT and mM organoids, we previously identified that aberrant expression of several developmental TFs led to enhancer reprogramming and endowed aggressive characteristics seen in PDA metastasis.[8, 25] The TFs, including Batf2, Foxa1, Gata5, Prrx2, Pax9, Trerf1, and En1, were highly expressed in mM organoids compared to their paired mT organoids (Figure 1B; Figure S1I, Supporting Information). Here, TRERF1 is linked to Patent ductus arteriosus.