EN1 has been shown to be a pro‐survival factor in brain development and associated with poor prognosis in multiple cancer types, such as adenoid cystic sarcoma, triple negative breast cancer (TNBC), nasopharyngeal carcinoma and osteosarcoma.[17, 18, 19, 20, 21, 22, 23] Our data showed that EN1 perturbations altered the expression of a number of genes involved in apoptosis‐, MYC‐, hypoxia‐ and E2F‐related pathways. Here, MYC is linked to osteosarcoma.