We further highlighted a novel mechanism underlying celastrol-induced cellular proliferation inhibition and apoptosis in pancreatic cancer cells by downregulating Claspin and Bcl-2 expression, which was due to the down-regulation of YTHDF3 induced by celastrol treatment and thereby reduced the interaction of YTHDF3 with Claspin and Bcl-2 mRNA. Here, YTHDF3 is linked to pancreatic neoplasm.